1CF3
GLUCOSE OXIDASE FROM APERGILLUS NIGER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 67.540, 67.540, 215.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.24300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gal |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.023 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.054 | 0.122 |
| Number of reflections | 40401 | |
| <I/σ(I)> | 21.5 | 6.9 |
| Completeness [%] | 87.7 | 78.7 |
| Redundancy | 2.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | Kalisz, H.M., (1990) J.Mol.Biol., 213, 207. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | glucose oxidase | 20 (mg/ml) | |
| 2 | 1 | drop | acetate | 50 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 1.6 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 5 | 1 | drop | octanetriol solution | saturated |
