1CBU

ADENOSYLCOBINAMIDE KINASE/ADENOSYLCOBINAMIDE PHOSPHATE GUANYLYLTRANSFERASE (COBU) FROM SALMONELLA TYPHIMURIUM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	263
Detector technology	AREA DETECTOR
Collection date	1997-09
Detector	SIEMENS HI-STAR
Spacegroup name	C 2 2 21
Unit cell lengths	96.400, 114.400, 106.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	18.500 *
Data reduction software	XDS
Data scaling software	XCALIBRE
Phasing software	HEAVY
Refinement software	TNT (4C)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.370
High resolution limit [Å]	2.300	2.300
Rmerge	0.043 *	0.210 *
Number of reflections	24136
<I/σ(I)>	16	1.7
Completeness [%]	88.5	64.4
Redundancy	3	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	5.5	4 *	PROTEIN WAS CRYSTALLIZED BY MICRO BATCH FROM 6% PEG 3350, 200 MM NACL AND 50 MM SUCCINATE, PH 5.5 AT 4 C., micro batch

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG3350	6 (%)
2	1	1		200 (mM)
3	1	1	succinate	50 (mM)
4	1	1	protein	5-8 (mg/ml)