1CBG

THE CRYSTAL STRUCTURE OF A CYANOGENIC BETA-GLUCOSIDASE FROM WHITE CLOVER (TRIFOLIUM REPENS L.), A FAMILY 1 GLYCOSYL-HYDROLASE

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1994-04-16
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	70.326, 70.326, 249.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.000 - 2.150
R-factor	0.189 *
Rwork	0.195
R-free	0.24700
RMSD bond length	0.010 *
RMSD bond angle	0.036 *
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.150
Rmerge	0.057
Number of reflections	30663
Completeness [%]	88.0
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *	20 *	Tolley, S.P., (1993) J.Mol.Biol., 229, 791. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulphate	48-52 (%(w/v))
2	1	reservoir	Tris-maleate	200 (mM)