1C5F
CRYSTAL STRUCTURE OF THE CYCLOPHILIN-LIKE DOMAIN FROM BRUGIA MALAYI COMPLEXED WITH CYCLOSPORIN A
Replaces: 1QTLExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.000, 100.160, 133.920 |
| Unit cell angles | 90.00, 93.75, 90.00 |
Refinement procedure
| Resolution | 47.100 - 2.470 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.320 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.140 | 2.530 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.120 | 0.372 |
| Total number of observations | 409247 * | |
| Number of reflections | 56200 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 77.5 * | 73.1 |
| Redundancy | 2.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 22 * | PEG 8000, CALCIUM ACETATE, METHANOL, MES, PH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 20 (%(w/v)) | |
| 3 | 1 | reservoir | cadmium acetate | 0.4 (M) | |
| 4 | 1 | reservoir | MES | 0.1 (M) |
