1C4Z
STRUCTURE OF AN E6AP-UBCH7 COMPLEX: INSIGHTS INTO THE UBIQUITINATION PATHWAY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-09-03 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.400, 112.700, 123.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.234 |
| Rwork | 0.237 |
| R-free | 0.29000 |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.707 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.055 | 0.413 |
| Total number of observations | 106238 * | |
| Number of reflections | 42425 | |
| <I/σ(I)> | 17 | |
| Completeness [%] | 89.8 * | 89.5 |
| Redundancy | 2.5 | 2.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG1500, MAGNESIUM CHLORIDE, MES-NA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 32 (mg/ml) | |
| 2 | 1 | reservoir | PEG1500 | 10 (%(w/v)) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | sodium MES | 0.1 (M) | pH6.5 |
