1C0G

CRYSTAL STRUCTURE OF 1:1 COMPLEX BETWEEN GELSOLIN SEGMENT 1 AND A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 228: Q228K/T229A/A230Y/E360H)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06-23
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.708
Spacegroup name	P 21 21 21
Unit cell lengths	56.862, 69.030, 181.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
Rwork	0.182
R-free	0.22300
RMSD bond length	0.011 *
RMSD bond angle	1.900 *
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.064	0.149
Total number of observations	161420 *
Number of reflections	45284
<I/σ(I)>	8
Completeness [%]	92.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	295	MES, SODIUM CHLORIDE, ATP, CALCIUM CHLORIDE, MAGNESIUM CHLORIDE, SODIUM AZIDE, DITHIOTHREITOL, PEG 6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
10	1	reservoir		150 (mM)
11	1	reservoir	ATP	0.1 (mM)
12	1	reservoir		0.1 (mM)
13	1	reservoir		0.1 (mM)
14	1	reservoir		1 (mM)
15	1	reservoir	dithiothreitol	10 (mM)
2	1	drop	imidazole-HCl	10 (mM)
3	1	drop		150 (mM)
4	1	drop	ATP	0.1 (mM)
5	1	drop		0.1 (mM)
6	1	drop		0.1 (mM)
7	1	drop		1 (mM)
8	1	drop	dithiothreitol	0.1 (mM)
9	1	reservoir	imidazole-HCl	50 (mM)