1BWB
HIV-1 PROTEASE (V82F/I84V) DOUBLE MUTANT COMPLEXED WITH SD146 OF DUPONT PHARMACEUTICALS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 278 |
| Spacegroup name | P 61 |
| Unit cell lengths | 62.800, 62.800, 83.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.900 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.079 * |
| Total number of observations | 99302 * |
| Number of reflections | 17416 |
| Completeness [%] | 99.0 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 18 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mg/ml) | |
| 2 | 1 | drop | acetate | 250 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 80-240 (mM) | |
| 4 | 1 | drop | inhibitor | 1000-fold molar excess |
