1BVO
DORSAL HOMOLOGUE GAMBIF1 BOUND TO DNA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-16 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 87.610, 87.610, 96.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.28800 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.041 * | 0.286 * |
| Total number of observations | 33776 * | |
| Number of reflections | 8927 | |
| <I/σ(I)> | 18.9 | 2.7 |
| Completeness [%] | 82.9 | 88.5 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 21 * | pH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 2-5 (%) | |
| 2 | 1 | reservoir | MES | 50 (mM) | |
| 3 | 1 | reservoir | 5 (mM) | ||
| 4 | 1 | reservoir | dithiothreitol | 3 (mM) |
