1BU2

X-RAY STRUCTURE OF A VIRAL CYCLIN FROM HERPESVIRUS SAIMIRI

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	100
Collection date	1995-11
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	37.920, 61.440, 130.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.242
Rwork	0.242
R-free	0.32400 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008 *
RMSD bond angle	19.700 *
Data reduction software	HKL
Data scaling software	SCALEPACK
Phasing software	CNS (0.3)
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.000
Rmerge	0.095 *	0.350 *
Total number of observations	23083 *
Number of reflections	6501
<I/σ(I)>	11.5
Completeness [%]	98.9	99.8 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7	22 *	pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.0-4.5 (mg/ml)
2	1	drop	EDTA	0.2 (mM)
3	1	drop	dithiothreitol	10 (mM)
4	1	reservoir	Na/K-phosphate	0.8 (M)
5	1	reservoir	PEG1500	4 (%)
6	1	reservoir	dithiothreitol	20 (mM)