1BJ4
RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (HUMAN)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 154.400, 154.400, 235.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.650 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.22600 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.680 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.102 | 0.659 * |
| Total number of observations | 500988 * | |
| Number of reflections | 49366 | |
| <I/σ(I)> | 22.2 | 1.9 |
| Completeness [%] | 97.8 | 25.9 * |
| Redundancy | 10.1 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 286 * | Renwick, S.B., (1998) Acta Cryst., D54, 1030. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 22-27 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 1.0 (M) | |
| 3 | 1 | reservoir | imidazole | 0.1 (M) |
