1BHN

NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1997-01-15
Detector	SIEMENS HI-STAR
Spacegroup name	P 21 21 21
Unit cell lengths	89.880, 92.110, 131.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.2 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PARTIALLY REFINED STRUCTURE OF THE B ISOFORM
RMSD bond length	0.021
RMSD bond angle	20.460 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	AMoRE
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.510
High resolution limit [Å]	2.400	2.400
Rmerge	0.122 *	0.384 *
Total number of observations	165667 *
Number of reflections	41872
<I/σ(I)>	6.8	1.4
Completeness [%]	79.0	60
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5		drop contains equal volume of the reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	(mg/ml)
2	1	drop	GTP	(mM)
3	1	drop	cGMP	(mM)
4	1	reservoir	sodium acetate	(mM)
5	1	reservoir	ammonium acetate	(mM)
6	1	reservoir	PEG4000	(%(w/v))