1BHG

HUMAN BETA-GLUCURONIDASE AT 2.6 A RESOLUTION

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-02-10
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 2
Unit cell lengths	95.100, 124.400, 134.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.530
R-factor	0.231
Rwork	0.231
R-free	0.31000
RMSD bond length	0.012
RMSD bond angle	1.900
Data reduction software	XENGEN ((ANDERSON)
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.600 *
Rmerge	0.078
Number of reflections	46839
Completeness [%]	93.2
Redundancy	4.71

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.1 (mg/ml)
2	1	drop	MPD	15 (%)
3	1	drop	Tris-HCl	5 (mM)
4	1	drop	sodium azide	0.01 (%(w/v))
5	1	reservoir	MPD	30 (%)