1BDG
HEXOKINASE FROM SCHISTOSOMA MANSONI COMPLEXED WITH GLUCOSE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 153 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 114.300, 114.300, 155.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1HKG AND YHK PI MUTANT P152K COMPLEX WITH GLC PROVIDED BY H. BARTUNIK |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.700 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.800 | |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.076 * | 0.330 |
| Total number of observations | 113216 * | |
| Number of reflections | 19765 | |
| <I/σ(I)> | 38 | 9 |
| Completeness [%] | 93.0 | 86 * |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.9-2.0 (M) | |
| 3 | 1 | reservoir | G6P | 2 (mM) | |
| 4 | 1 | reservoir | PEG400 | 2.4 (%) | |
| 5 | 1 | reservoir | HEPES | 100 (mM) |
