1AOW

ANNEXIN IV

Experimental procedure

Temperature [K]	100
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1997-02
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	118.560, 118.560, 82.233
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 * - 2.990 *
R-factor	0.19
Rwork	0.190
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ann
RMSD bond length	0.019
RMSD bond angle	3.570
Data reduction software	XDS
Data scaling software	MARSCALE
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	3.120
High resolution limit [Å]	2.990 *	2.990
Rmerge	0.049	0.070
Number of reflections	8114
<I/σ(I)>	36	10
Completeness [%]	93.0 *	87 *
Redundancy	5.6	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5		SITTING DROP, FROM A PROTEIN SOLUTION 10 MG/ML, IN THE PRESENCE OF 1.4 M AMMONIUM SULFATE, 10 MM BETA-MERCAPTOETHANOL, PH=5.0, vapor diffusion - sitting drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium sulfate	1 (M)
3	1	drop	Tris-HCl	50 (mM)
4	1	reservoir	ammonium sulfate	1.4 (M)
5	1	reservoir	2-mercaptoethanol	10 (mM)
6	1	reservoir	sodium citrate	0.1 (M)