1ANX

THE CRYSTAL STRUCTURE OF A NEW HIGH-CALCIUM FORM OF ANNEXIN V

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	91.200, 91.300, 36.200
Unit cell angles	82.40, 82.40, 118.20

Refinement procedure

Resolution	12.000 - 1.900
R-factor	0.198
RMSD bond length	0.015
RMSD bond angle	0.050
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	12.000 *
High resolution limit [Å]	1.900 *
Rmerge	0.040 *
Total number of observations	184680 *
Number of reflections	74259 *
Completeness [%]	94.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	calcium	10-60 (mM)
4	1	reservoir	ammonium sulfate	1.8-2 (M)
5	1	reservoir	Tris-HCl	50 (mM)
6	1	drop	phospholipid		or a mixture of 0.1 %(w/v) agarose or 0.1 %(w/v) sucrose