1AKN
STRUCTURE OF BILE-SALT ACTIVATED LIPASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 94.390, 94.390, 144.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.216 * |
| Rwork | 0.211 |
| R-free | 0.28300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ACE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 * | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.066 | 0.391 * |
| Number of reflections | 18614 | |
| <I/σ(I)> | 13.8 | 2.8 |
| Completeness [%] | 98.4 | 99.8 * |
| Redundancy | 2.53 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 20 * | pH 7. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12.5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 0.9 (M) | |
| 3 | 1 | drop | isopropanol | 2.5 (%) | |
| 4 | 1 | drop | zwittergent 3-12 | 0.5 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 6 | 1 | reservoir | isopropanol | 5 (%) | |
| 7 | 1 | reservoir | zwittergent 3-12 | 1 (mM) |
