1AIO
CRYSTAL STRUCTURE OF A DOUBLE-STRANDED DNA CONTAINING THE MAJOR ADDUCT OF THE ANTICANCER DRUG CISPLATIN
Replaces: 1GPGExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.270, 35.460, 47.010 |
| Unit cell angles | 79.81, 84.75, 82.79 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25100 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.062 * |
| Number of reflections | 5797 |
| Completeness [%] | 97.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 * | 4 * | pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | [CO(NH3)6]CL3 | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.2 (mM) | |
| 2 | 1 | drop | cacodylate acid | 52 (mM) | |
| 3 | 1 | drop | 15 (mM) | ||
| 4 | 1 | drop | 6 (mM) | ||
| 5 | 1 | drop | MPD | 3 (%) | |
| 6 | 1 | reservoir | MPD | 5 (%) |
