1A8G

HIV-1 PROTEASE IN COMPLEX WITH SDZ283-910

Experimental procedure

Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	293
Detector technology	DIFFRACTOMETER
Collection date	1993-09
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 61
Unit cell lengths	63.100, 63.100, 83.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.15
Rwork	0.150
R-free	0.24400
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	9hvp
RMSD bond length	0.016
RMSD bond angle	28.200 *
Data reduction software	MADNES
Data scaling software	PROCOR
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.670
High resolution limit [Å]	2.500	2.500
Rmerge	0.087 *	0.270 *
Total number of observations	22790 *
Number of reflections	5696
<I/σ(I)>	12	3
Completeness [%]	81.0	77
Redundancy	4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	inhibitor		0.006mM
2	1	drop	ammonium sulfate	5-25 (%(v/v))	0.006mM