1A81
CRYSTAL STRUCTURE OF THE TANDEM SH2 DOMAIN OF THE SYK KINASE BOUND TO A DUALLY TYROSINE-PHOSPHORYLATED ITAM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.500, 146.900, 91.500 |
| Unit cell angles | 90.00, 97.60, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.31700 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.85) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.051 * | |
| Total number of observations | 202021 * | |
| Number of reflections | 40670 | |
| Completeness [%] | 89.8 | 74.1 |
| Redundancy | 5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 10% (W/V) PEG800, 10% (V/V) PEG 200, 0.1M TRIS HCL PH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17.5 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 4.5-6 (%(w/v)) | |
| 3 | 1 | drop | PEG200 | 5 (%(v/v)) | |
| 4 | 1 | drop | Tris-HCl | 0.05 (M) | |
| 5 | 1 | reservoir | PEG8000 | 9-12 (%(w/v)) | |
| 6 | 1 | reservoir | PEG200 | 10 (%(v/v)) | |
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) |
