1A7H
GAMMA S CRYSTALLIN C-TERMINAL DOMAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 62.250, 62.250, 170.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.560 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | GAMMA B C-TERMINAL |
| RMSD bond length | 0.009 |
| RMSD bond angle | 26.130 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.640 | 2.650 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.035 | 0.058 |
| Total number of observations | 33311 * | |
| Number of reflections | 6716 | |
| <I/σ(I)> | 14.7 | 11.5 |
| Completeness [%] | 98.6 | 97.9 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 20 * | PROTEIN CONCENTRATION 5MGS/ML. 0.1M NA-CITRATE, PH 5.0, 20-22%(W/V) PEG8K, 2% 1,4-DIOXANE, AT ROOM-TEMPERATURE. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Mes/NaOH | 25 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 11 (%(w/v)) | |
| 4 | 1 | reservoir | Na-citrate |
