1A6R
GAL6 (YEAST BLEOMYCIN HYDROLASE) MUTANT C73A
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 150.676, 150.676, 90.163 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.050 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 21.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.077 | 0.164 |
| Total number of observations | 367565 * | |
| Number of reflections | 37272 | |
| <I/σ(I)> | 58 | 31 |
| Completeness [%] | 97.3 | 99.2 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.5 * | PROTEIN WAS CRYSTALLIZED FROM AMMONIUM SULFATE AND PEG 4K., pH 9.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | bleomycin | 0.5 (mM) | |
| 3 | 1 | drop | Tris-HCl | 25 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 10 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 0.45 (M) | |
| 6 | 1 | reservoir | Tris | 0.1 (M) |
