1A6Q
CRYSTAL STRUCTURE OF THE PROTEIN SERINE/THREONINE PHOSPHATASE 2C AT 2 A RESOLUTION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BL19 |
| Synchrotron site | ESRF |
| Beamline | BL19 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 91.020, 91.020, 105.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.370 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.82) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.089 |
| Total number of observations | 215022 * |
| Number of reflections | 33176 |
| <I/σ(I)> | 12.6 |
| Completeness [%] | 95.5 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5 | 100 * | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | potassium phosphate | 50 (mM) | |
| 2 | 1 | reservoir | PEG8000 | 8-12 (%) | |
| 3 | 1 | reservoir | glycerol | 15 (%(v/v)) | |
| 4 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 5 | 1 | drop | Tris-HCl | 10 (mM) | |
| 6 | 1 | drop | 50 (mM) | ||
| 7 | 1 | drop | 1 (mM) | ||
| 8 | 1 | drop | dithiothreitol | 2 (mM) | |
| 9 | 1 | drop | protein | 15 (mg/ml) |
