1A22
HUMAN GROWTH HORMONE BOUND TO SINGLE RECEPTOR
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1991-05 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.700, 67.700, 228.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.600 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hhr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.063 | 0.116 |
| Total number of observations | 51354 * | |
| Number of reflections | 14489 | 1552 * |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 85.0 | 78 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | Ultsch, M., (1993) J.Mol.Biol., 231, 1133. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 30 (%sat) | |
| 2 | 1 | drop | 120 (mM) | ||
| 3 | 1 | drop | sodium acetate | 20 (mM) | |
| 4 | 1 | drop | PMSF | 1 (mM) | |
| 5 | 1 | drop | Bis-Tris | 0.1 (M) | |
| 6 | 1 | drop | ammonium sulfate | 10 (%sat) | |
| 7 | 1 | drop | MPD | 1 (%(v/v)) |
