13HA

The structure of Fab_C1 in complex with CD30

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-02-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919907
Spacegroup name	P 31 2 1
Unit cell lengths	72.150, 72.150, 220.042
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.560 - 2.000
R-factor	0.1909
Rwork	0.189
R-free	0.22230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.935
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	220.040	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.165	2.421
Rmeas	0.174	2.557
Rpim	0.053	0.808
Number of reflections	46203	2246
<I/σ(I)>	12	0.8
Completeness [%]	100.0	99
Redundancy	20	18.6
CC(1/2)	0.998	0.394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	20% PEG3350 and 0.1M Bis-tris methane, pH 5.5. Cryoprotected with the same buffer plus 30% ethylene glycol