12UQ
BCL11B ZF2-3 in Complex with a DNA Sequence Containing Two Binding Sites (Motifs TGTCCC and TGGCCT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2026-02-06 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.919901 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.290, 65.854, 46.809 |
| Unit cell angles | 90.00, 97.21, 90.00 |
Refinement procedure
| Resolution | 35.300 - 3.290 |
| R-factor | 0.2692 |
| Rwork | 0.268 |
| R-free | 0.29540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.489 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (2.0_5885) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.300 | 3.200 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Number of reflections | 13376 | 636 |
| <I/σ(I)> | 2.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.988 | 0.768 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 292 | 26 % w/v PEG 2000 MME, 0.1 M Bis-Tris pH 5.8 |
