12UP

BCL11B ZF2-3 in Complex with a DNA Sequence Observed in the Human Globin Locus Containing Motif TGGCCA (Oligo 4-2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-08-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919716
Spacegroup name	P 1
Unit cell lengths	35.167, 38.701, 59.860
Unit cell angles	97.11, 98.65, 99.70

Refinement procedure

Resolution	34.150 - 2.830
R-factor	0.2369
Rwork	0.234
R-free	0.28720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.590
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (2.0_5885)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.150	2.880
High resolution limit [Å]	2.830	2.830
Number of reflections	14314	658
<I/σ(I)>	3.4
Completeness [%]	99.1
Redundancy	3.6
CC(1/2)	0.996	0.453

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	30% w/v Polyethylene glycol monomethyl ether 2,000, 0.15 M Potassium bromide