12KH

The Condensation Domain from Coprococcus Eutactus, OaaC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-23
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	H 3
Unit cell lengths	122.550, 122.550, 233.753
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.750 - 2.150
R-factor	0.2032
Rwork	0.201
R-free	0.23890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.563
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.750	2.180
High resolution limit [Å]	2.150	2.150
Rmerge	0.097	0.845
Rmeas	0.106	0.934
Rpim	0.042	0.392
Number of reflections	10103	1830
<I/σ(I)>	8.65	1.49
Completeness [%]	99.6	98.59
Redundancy	6.2	5.5
CC(1/2)	0.996	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	296.15	OaaC at 12 mg/mL and a crystallization cocktail of 0.1 M CHES, NaOH, pH 9, 3mM AVA, 35% PEG 3000 w/v, and a protein:cocktail drop volume ration of 2:1.