12E8

2E8 FAB FRAGMENT

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	125
Detector technology	AREA DETECTOR
Collection date	1996-05
Detector	ADSC
Spacegroup name	P 1 21 1
Unit cell lengths	66.560, 65.570, 103.220
Unit cell angles	90.00, 97.04, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.221
Rwork	0.221
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 6FAB AND 1FLR
RMSD bond length	0.011
RMSD bond angle	28.400 *
Data reduction software	UCSD (DATA PROCESSING SOFTWARE)
Data scaling software	UCSD
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.000
High resolution limit [Å]	1.850	1.850
Rmerge	0.085	0.390
Total number of observations	237795 *
Number of reflections	63078
<I/σ(I)>	6	1.6
Completeness [%]	84.0	82
Redundancy	3.8	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6		23% PEG 4000 (W/V), 0.10 M SODIUM CITRATE, AND 0.2 M AMMONIUM ACETATE (PH 5.6). CRYSTALS (0.2 X 0.2 X 0.5 MM) WERE OBTAINED IN 4-6 WEEKS.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	reservoir	PEG4000	23 (%(w/v))
3	1	reservoir	sodium citrate	0.10 (M)
4	1	reservoir	ammonium acetate	0.2 (M)	pH5.6