12DP

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with sparsomycin, mRNA, and aminoacylated initiator P-site fMet-tRNAmet at 2.95A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-15
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	209.520, 449.290, 620.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	173.670 - 2.950
R-factor	0.2105
Rwork	0.208
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.009
RMSD bond angle	1.383
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	173.670	173.670	3.030
High resolution limit [Å]	2.950	13.190	2.950
Rmerge	0.252	0.042	2.053
Rmeas	0.273	0.046	2.230
Total number of observations	8221155
Number of reflections	1213557	14313	89270
<I/σ(I)>	7.48	33.99	0.98
Completeness [%]	99.9	99.1	100
Redundancy	6.774	6.779	6.512
CC(1/2)	0.997	0.999	0.262

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME