12E8
2E8 FAB FRAGMENT
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 125 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-05 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.560, 65.570, 103.220 |
| Unit cell angles | 90.00, 97.04, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 6FAB AND 1FLR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 28.400 * |
| Data reduction software | UCSD (DATA PROCESSING SOFTWARE) |
| Data scaling software | UCSD |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.000 | |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.085 | 0.390 |
| Total number of observations | 237795 * | |
| Number of reflections | 63078 | |
| <I/σ(I)> | 6 | 1.6 |
| Completeness [%] | 84.0 | 82 |
| Redundancy | 3.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 23% PEG 4000 (W/V), 0.10 M SODIUM CITRATE, AND 0.2 M AMMONIUM ACETATE (PH 5.6). CRYSTALS (0.2 X 0.2 X 0.5 MM) WERE OBTAINED IN 4-6 WEEKS. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 23 (%(w/v)) | |
| 3 | 1 | reservoir | sodium citrate | 0.10 (M) | |
| 4 | 1 | reservoir | ammonium acetate | 0.2 (M) | pH5.6 |
