11QF

I-PnoMI with solubilizing mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	95
Detector technology	PIXEL
Collection date	2024-12-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97
Spacegroup name	I 1 2 1
Unit cell lengths	76.945, 168.234, 76.957
Unit cell angles	90.00, 93.65, 90.00

Refinement procedure

Resolution	46.680 - 1.870
R-factor	0.1843
Rwork	0.183
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.138
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.680	1.910
High resolution limit [Å]	1.870	1.870
Rmerge	0.071	0.752
Rmeas	0.077	0.826
Rpim	0.029	0.335
Number of reflections	78761	4100
<I/σ(I)>	14.3	2.1
Completeness [%]	98.2	90.3
Redundancy	6.8	5.8
CC(1/2)	0.998	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	21% w/v PEG 3000 0.2 M Ammonium citrate tribasic 50 mM Tris pH 7.5