11EX

Crystal structure of hIgG1-Fc in complex with FcRL5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97905
Spacegroup name	P 31 2 1
Unit cell lengths	138.716, 138.716, 130.511
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	88.390 - 3.420
R-factor	0.2575
Rwork	0.255
R-free	0.29870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.489
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.390	3.480
High resolution limit [Å]	3.420	3.420
Rmerge	0.672	3.570
Rmeas	0.689	3.656
Rpim	0.151	0.785
Total number of observations	414740	20961
Number of reflections	20062	964
<I/σ(I)>	3.6	0.6
Completeness [%]	99.9
Redundancy	20.7	21.7
CC(1/2)	0.956	0.154

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298.15	0.1MES, pH6.5, 25%(w/v) PEG 8000