11CF
CHAP domain of Staphylococcus aureus-specific lysin L1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.120, 57.430, 63.050 |
| Unit cell angles | 86.07, 85.22, 83.99 |
Refinement procedure
| Resolution | 36.640 - 1.250 |
| R-factor | 0.18783 |
| Rwork | 0.187 |
| R-free | 0.19894 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.021 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.640 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.042 | 0.234 |
| Rpim | 0.042 | 0.234 |
| Number of reflections | 165929 | 12531 |
| <I/σ(I)> | 14.8 | 5 |
| Completeness [%] | 94.5 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.998 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 mM HEPES 7.4, 2 mM TCEP, 150 mM sodium chloride |
