11BG

A POTENTIAL ALLOSTERIC SUBSITE GENERATED BY DOMAIN SWAPPING IN BOVINE SEMINAL RIBONUCLEASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-10-15
Detector	MAC Science DIP-2030
Spacegroup name	P 2 21 21
Unit cell lengths	36.400, 66.700, 107.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 1.900
R-factor	0.189 *
Rwork	0.189
Structure solution method	OTHER
Starting model (for MR)	1bsr
RMSD bond length	0.016
RMSD bond angle	25.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.072	0.245 *
Total number of observations	97315 *
Number of reflections	20713
Completeness [%]	96.7	90 *
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.8	20 *	nucleotide to protein molar ratio of 8:1 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	62-64 (%sat)
2	1	1	MPD	8-10 (%(v/v))
3	1	1	protein	10 (mg/ml)