11BA
BINDING OF A SUBSTRATE ANALOGUE TO A DOMAIN SWAPPING PROTEIN IN THE COMPLEX OF BOVINE SEMINAL RIBONUCLEASE WITH URIDYLYL-2',5'-ADENOSINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.450, 60.640, 50.750 |
Unit cell angles | 90.00, 117.35, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.060 |
Rwork | 0.184 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bsr |
RMSD bond length | 0.019 |
RMSD bond angle | 27.100 * |
Data reduction software | bioteX |
Data scaling software | bioteX |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.250 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.059 | |
Number of reflections | 15798 | |
Completeness [%] | 92.0 | 91 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 4.8 | 20 * | pH 4.8 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | ammonium sulfate | 62-64 (%sat) | |
2 | 1 | 1 | MPD | 8-10 (%(v/v)) | |
3 | 1 | 1 | protein | 10 (mg/ml) | |
4 | 1 | 1 | nucleotide/protein | molar ratio of 8:1 |