11BA
BINDING OF A SUBSTRATE ANALOGUE TO A DOMAIN SWAPPING PROTEIN IN THE COMPLEX OF BOVINE SEMINAL RIBONUCLEASE WITH URIDYLYL-2',5'-ADENOSINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.450, 60.640, 50.750 |
| Unit cell angles | 90.00, 117.35, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.060 |
| Rwork | 0.184 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bsr |
| RMSD bond length | 0.019 |
| RMSD bond angle | 27.100 * |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.250 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.059 | |
| Number of reflections | 15798 | |
| Completeness [%] | 92.0 | 91 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 4.8 | 20 * | pH 4.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 62-64 (%sat) | |
| 2 | 1 | 1 | MPD | 8-10 (%(v/v)) | |
| 3 | 1 | 1 | protein | 10 (mg/ml) | |
| 4 | 1 | 1 | nucleotide/protein | molar ratio of 8:1 |
