10ZJ

Structure of Cbl-B bound to compound 8

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-17
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	57.190, 74.120, 97.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.290 - 1.770
R-factor	0.2269
Rwork	0.225
R-free	0.26880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.399
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19-4092_final)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.290	1.830
High resolution limit [Å]	1.770	1.770
Number of reflections	32919	902
<I/σ(I)>	11.5
Completeness [%]	95.3
Redundancy	2
CC(1/2)	0.990	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.01M MgCl2, 10% PEG6000