10ZF

Structure of c-Cbl bound to compound 33

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-27
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	101.720, 49.860, 83.872
Unit cell angles	90.00, 92.06, 90.00

Refinement procedure

Resolution	30.350 - 2.390
R-factor	0.2011
Rwork	0.198
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.465
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.350	2.630
High resolution limit [Å]	2.390	2.390
Rmerge	0.120
Number of reflections	11608	699
<I/σ(I)>	6.2	1.5
Completeness [%]	88.5
Redundancy	3.4
CC(1/2)	0.990	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M NaCH3COO, 0.1M Tris pH8.5, 16% PEG4000