10OU

FGFR2 mutant D650V with compound 12

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97625
Spacegroup name	P 31 2 1
Unit cell lengths	96.909, 96.909, 145.896
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.060 - 1.770
R-factor	0.17384
Rwork	0.172
R-free	0.21260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.097
Data reduction software	SAINT
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.060	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.094	2.612
Number of reflections	80528	4334
<I/σ(I)>	15.4	1.5
Completeness [%]	99.9	98.7
Redundancy	20.2	18.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	293	22% PEG 4,000, 200 mM Ammonium Sulfate, 100 mM Sodium Citrate pH 5.6