10JG
Structure of Human Argonaute2-miR200b RISC bound to 13nt long target in open conformation
EM specimen
| Vitrification | Yes |
| Instrument | LEICA PLUNGER |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Number of Particle | 817861 |
| Software | Warp,cryoSPARC,PHENIX,EPU,UCSF ChimeraX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
