10GI

Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from Babesia bovis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRIUS BEAMLINE MANACA
Synchrotron site	LNLS SIRIUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-12-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9772
Spacegroup name	P 31 2 1
Unit cell lengths	85.614, 85.614, 155.063
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.140 - 3.120
R-factor	0.2926
Rwork	0.290
R-free	0.34050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.385
Data reduction software	DIALS (3.14.2)
Data scaling software	Aimless (0.8.2)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.201	3.512
High resolution limit [Å]	3.118	3.118
Rmerge	0.394	3.951
Rmeas	0.405	4.054
Rpim	0.094	0.900
Number of reflections	9849	1414
<I/σ(I)>	7.2	1.2
Completeness [%]	90.9	63.7
Redundancy	19	19.9
CC(1/2)	0.993	0.486

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1 mol/L TRIS pH 8.5, 25% (m/V) PEG 3350