10DC
H-Ras GTPase R68A bound to GppNHp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-01-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 41 |
| Unit cell lengths | 69.896, 69.896, 33.321 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.300 - 2.080 |
| R-factor | 0.1697 |
| Rwork | 0.165 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.553 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.21.2_5419) |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.300 | 2.200 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.107 | 0.413 |
| Number of reflections | 9861 | 1385 |
| <I/σ(I)> | 27.5 | |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.982 | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M Calcium Acetate, 20% w/v PEG 3350, 0.1% N-octyl glucopyranoside |
