10AK
Crystal Structure of Human WRN helicase with compound 4
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.293, 58.216, 131.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.810 - 1.370 |
| R-factor | 0.2037 |
| Rwork | 0.203 |
| R-free | 0.21414 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.326 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC ((Version 1.1.7)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.810 | 1.392 |
| High resolution limit [Å] | 1.368 | 1.368 |
| Rmerge | 0.050 | 1.308 |
| Number of reflections | 95235 | 4732 |
| <I/σ(I)> | 18 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 290 | protocol was established using PROTEROS Standard Protocols |
