105M
SPERM WHALE MYOGLOBIN N-BUTYL ISOCYANIDE AT PH 9.0
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-07 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.720, 30.061, 35.191 |
| Unit cell angles | 90.00, 105.76, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.020 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SPERM WHALE MYOGLOBIN NBNC WITHOUT LIGAND |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 2.030 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.071 | 0.225 |
| Number of reflections | 7987 | |
| Completeness [%] | 90.7 | 90.9 |
| Redundancy | 1.82 | 1.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 3.0 M AMMONIUM SULFATE, UNBUFFERED, pH 9.0 |
