0.5-1.0 mM [U-13C; U-15N] N-ClpCR, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.5-1.0 mM [U-15N] N-ClpCR, 90% H2O/10% D2O
90% H2O/10% D2O
試料
単位
構成要素
Isotopic labeling
Conc. range (mg/ml)
Solution-ID
mM
N-ClpCR
[U-13C; U-15N]
0.5-1.0
1
mM
N-ClpCR
[U-15N]
0.5-1.0
2
試料状態
イオン強度: 50 / pH: 5.5 / 圧: ambient / 温度: 305 K
-
NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Varian INOVA
Varian
INOVA
800
1
Varian INOVA
Varian
INOVA
600
2
Varian INOVA
Varian
INOVA
500
3
-
解析
NMR software
名称
バージョン
開発者
分類
ARIA
1.2
Linge, O'DonoghueandNilges
chemicalshiftassignment
ARIA
1.2
Linge, O'DonoghueandNilges
構造決定
Amber
9
Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... andKollm
精密化
relax
1.2
d'AuvergneandGooley
model-free analysis of relaxation data
CNS
1.1
Brunger, Adams, Clore, Gros, NilgesandRead
構造決定
精密化
手法: simulated annealing / ソフトェア番号: 1 詳細: 200 initial structures calculated with ARIA 1.2/CNS 1.1 using NOE, dihedral, and RDC restraints, 100 lowest-energy structures were refined in AMBER 9 using the GB solvent model, 30 lowest- ...詳細: 200 initial structures calculated with ARIA 1.2/CNS 1.1 using NOE, dihedral, and RDC restraints, 100 lowest-energy structures were refined in AMBER 9 using the GB solvent model, 30 lowest-energy structures were deposited
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 30