+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1f45 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
タイトル | HUMAN INTERLEUKIN-12 | |||||||||
要素 |
| |||||||||
キーワード | CYTOKINE/CYTOKINE / interleukin (インターロイキン) / cytokine (サイトカイン) / CYTOKINE-CYTOKINE COMPLEX | |||||||||
機能・相同性 | 機能・相同性情報 interleukin-12 beta subunit binding / interleukin-27 binding / late endosome lumen / interleukin-12 alpha subunit binding / interleukin-12 complex / interleukin-23 complex / regulation of tyrosine phosphorylation of STAT protein / natural killer cell activation involved in immune response / positive regulation of dendritic cell chemotaxis / positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target ...interleukin-12 beta subunit binding / interleukin-27 binding / late endosome lumen / interleukin-12 alpha subunit binding / interleukin-12 complex / interleukin-23 complex / regulation of tyrosine phosphorylation of STAT protein / natural killer cell activation involved in immune response / positive regulation of dendritic cell chemotaxis / positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target / negative regulation of vascular endothelial growth factor signaling pathway / negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis / positive regulation of tissue remodeling / positive regulation of lymphocyte proliferation / positive regulation of activation of Janus kinase activity / 有性生殖 / positive regulation of T-helper 1 type immune response / positive regulation of smooth muscle cell apoptotic process / positive regulation of NK T cell activation / positive regulation of mononuclear cell proliferation / interleukin-12 receptor binding / positive regulation of memory T cell differentiation / natural killer cell activation / T-helper cell differentiation / negative regulation of interleukin-17 production / positive regulation of osteoclast differentiation / Interleukin-23 signaling / positive regulation of T-helper 17 type immune response / positive regulation of NK T cell proliferation / interleukin-12-mediated signaling pathway / positive regulation of granulocyte macrophage colony-stimulating factor production / Interleukin-12 signaling / Interleukin-35 Signalling / response to UV-B / cytokine receptor activity / positive regulation of natural killer cell activation / T-helper 1 type immune response / negative regulation of interleukin-10 production / positive regulation of natural killer cell mediated cytotoxicity / cytokine binding / positive regulation of activated T cell proliferation / defense response to protozoan / positive regulation of interleukin-17 production / Interleukin-10 signaling / positive regulation of natural killer cell proliferation / positive regulation of interleukin-10 production / negative regulation of protein secretion / positive regulation of cell adhesion / positive regulation of T-helper 17 cell lineage commitment / T cell proliferation / extrinsic apoptotic signaling pathway / positive regulation of tyrosine phosphorylation of STAT protein / regulation of cytokine production / negative regulation of inflammatory response to antigenic stimulus / positive regulation of defense response to virus by host / positive regulation of T cell proliferation / positive regulation of interleukin-12 production / cytokine activity / negative regulation of smooth muscle cell proliferation / response to virus / growth factor activity / cellular response to virus / positive regulation of inflammatory response / positive regulation of T cell mediated cytotoxicity / cellular response to type II interferon / cytokine-mediated signaling pathway / positive regulation of non-canonical NF-kappaB signal transduction / 遊走 / positive regulation of type II interferon production / positive regulation of tumor necrosis factor production / defense response to virus / Interleukin-4 and Interleukin-13 signaling / cellular response to lipopolysaccharide / defense response to Gram-negative bacterium / response to lipopolysaccharide / receptor complex / defense response to Gram-positive bacterium / 免疫応答 / protein heterodimerization activity / 小胞体 / external side of plasma membrane / protein-containing complex binding / extracellular space / extracellular region / identical protein binding / 細胞質基質 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | X線回折 / シンクロトロン / 解像度: 2.8 Å | |||||||||
データ登録者 | Yoon, C. / Johnston, S.C. / Tang, J. / Tobin, J.F. / Somers, W.S. | |||||||||
引用 | ジャーナル: EMBO J. / 年: 2000 タイトル: Charged residues dominate a unique interlocking topography in the heterodimeric cytokine interleukin-12. 著者: Yoon, C. / Johnston, S.C. / Tang, J. / Stahl, M. / Tobin, J.F. / Somers, W.S. | |||||||||
履歴 |
|
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
---|
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1f45.cif.gz | 93.6 KB | 表示 | PDBx/mmCIF形式 |
---|---|---|---|---|
PDB形式 | pdb1f45.ent.gz | 75 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1f45.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/f4/1f45 ftp://data.pdbj.org/pub/pdb/validation_reports/f4/1f45 | HTTPS FTP |
---|
-関連構造データ
-リンク
-集合体
登録構造単位 |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
単位格子 |
|
-要素
#1: タンパク質 | 分子量: 34739.945 Da / 分子数: 1 / 断片: RESIDUES 23-328 / 変異: A150F/A151N / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / プラスミド: PEMC / 細胞株 (発現宿主): CHO 発現宿主: Cricetulus griseus (モンゴルキヌゲネズミ) 参照: UniProt: P29460 |
---|---|
#2: タンパク質 | 分子量: 22568.154 Da / 分子数: 1 / 断片: RESIDUES 23-219 / 変異: T191M / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / プラスミド: PEMC / 細胞株 (発現宿主): CHO 発現宿主: Cricetulus griseus (モンゴルキヌゲネズミ) 参照: UniProt: P29459 |
#3: 多糖 | alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose |
#4: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 2 |
---|
-試料調製
結晶 | マシュー密度: 3.86 Å3/Da / 溶媒含有率: 68.14 % | ||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
結晶化 | 温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5 詳細: 12% MPD, 650mM NaCl, 100mM Na Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 温度: 18 ℃ | ||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
|
-データ収集
回折 | 平均測定温度: 100 K |
---|---|
放射光源 | 由来: シンクロトロン / サイト: NSLS / ビームライン: X4A / 波長: 0.99987 |
検出器 | タイプ: RIGAKU RAXIS / 検出器: IMAGE PLATE / 日付: 1997年1月1日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.99987 Å / 相対比: 1 |
反射 | 解像度: 2.8→12 Å / Num. all: 21109 / Num. obs: 21109 / % possible obs: 96 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / 冗長度: 5.4 % / Biso Wilson estimate: 40 Å2 / Rmerge(I) obs: 0.066 / Net I/σ(I): 25 |
反射 シェル | 解像度: 2.8→2.9 Å / 冗長度: 3.2 % / Rmerge(I) obs: 0.348 / % possible all: 96 |
反射 | *PLUS |
反射 シェル | *PLUS 最高解像度: 2.8 Å / 最低解像度: 2.9 Å / % possible obs: 96 % |
-解析
ソフトウェア |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
精密化 | 解像度: 2.8→12 Å / σ(F): 0 / σ(I): 0 / 立体化学のターゲット値: Engh & Huber
| ||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2.8→12 Å
| ||||||||||||||||||||
拘束条件 |
| ||||||||||||||||||||
ソフトウェア | *PLUS 名称: CNS / 分類: refinement | ||||||||||||||||||||
精密化 | *PLUS 最高解像度: 2.8 Å / 最低解像度: 12 Å / σ(F): 0 | ||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||
拘束条件 | *PLUS タイプ: c_angle_deg / Dev ideal: 2.2 |