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Yorodumi- PDB-4ylb: Crystal Structure of A102D mutant of hsp14.1 from Sulfolobus solf... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4ylb | ||||||
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Title | Crystal Structure of A102D mutant of hsp14.1 from Sulfolobus solfatataricus P2 | ||||||
Components | Heat shock protein Hsp20Heat shock response | ||||||
Keywords | CHAPERONE / Small heat shock protein / Molecular chaperone / SsHsp14.1 | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Sulfolobus solfataricus (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.501 Å | ||||||
Authors | Liu, L. / Chen, J.Y. / Yun, C.H. | ||||||
Citation | Journal: Structure / Year: 2015 Title: Active-State Structures of a Small Heat-Shock Protein Revealed a Molecular Switch for Chaperone Function Authors: Liu, L. / Chen, J.Y. / Yang, B. / Wang, F.H. / Wang, Y.H. / Yun, C.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4ylb.cif.gz | 110.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4ylb.ent.gz | 86.6 KB | Display | PDB format |
PDBx/mmJSON format | 4ylb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yl/4ylb ftp://data.pdbj.org/pub/pdb/validation_reports/yl/4ylb | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 14520.835 Da / Num. of mol.: 4 / Mutation: A102D Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus solfataricus (strain 98/2) (archaea) Strain: 98/2 / Gene: Ssol_0413 / Production host: Escherichia coli (E. coli) / References: UniProt: D0KNS6 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.11 Å3/Da / Density % sol: 60.48 % |
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Crystal grow | Temperature: 293 K / Method: evaporation Details: magnesium sulfate heptahydrate, polyethylene glycol 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 0.97913 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Mar 19, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97913 Å / Relative weight: 1 |
Reflection | Resolution: 2.49→50 Å / Num. obs: 24402 / % possible obs: 96.6 % / Redundancy: 5.7 % / Net I/σ(I): 21.5 |
-Processing
Software |
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Refinement | Resolution: 2.501→42.589 Å / SU ML: 0.35 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 26.72 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.501→42.589 Å
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Refine LS restraints |
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LS refinement shell |
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