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- SASDEP4: Protein tyrosine phosphatase SHP2 with E76K activating mutation -

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Basic information

Entry
Database: SASBDB / ID: SASDEP4
SampleProtein tyrosine phosphatase SHP2 with E76K activating mutation
  • Tyrosine-protein phosphatase non-receptor type 11 E76K (protein), SHP2 E76K, Homo sapiens
Function / homologyprotein-tyrosine-phosphatase / Isoform 1 of Tyrosine-protein phosphatase non-receptor type 11
Function and homology information
Biological speciesHomo sapiens (human)
CitationDate: 2018 Dec
Title: Mechanism of activating mutations and allosteric drug inhibition of the phosphatase SHP2
Authors: Pádua R / Sun Y / Marko I / Pitsawong W / Stiller J / Otten R
Contact author
  • RIcardo Padua (Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2377
Type: atomic / Chi-square value: 2.53576105228
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Protein tyrosine phosphatase SHP2 with E76K activating mutation
Specimen concentration: 0.76-12.20
BufferName: 50 mM ADA, 2 mM TCEP / pH: 6.5
Entity #1293Name: SHP2 E76K / Type: protein
Description: Tyrosine-protein phosphatase non-receptor type 11 E76K
Formula weight: 60.911 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q06124-2
Sequence: GSGMTSRRWF HPNITGVEAE NLLLTRGVDG SFLARPSKSN PGDFTLSVRR NGAVTHIKIQ NTGDYYDLYG GEKFATLAKL VQYYMEHHGQ LKEKNGDVIE LKYPLNCADP TSERWFHGHL SGKEAEKLLT EKGKHGSFLV RESQSHPGDF VLSVRTGDDK GESNDGKSKV ...Sequence:
GSGMTSRRWF HPNITGVEAE NLLLTRGVDG SFLARPSKSN PGDFTLSVRR NGAVTHIKIQ NTGDYYDLYG GEKFATLAKL VQYYMEHHGQ LKEKNGDVIE LKYPLNCADP TSERWFHGHL SGKEAEKLLT EKGKHGSFLV RESQSHPGDF VLSVRTGDDK GESNDGKSKV THVMIRCQEL KYDVGGGERF DSLTDLVEHY KKNPMVETLG TVLQLKQPLN TTRINAAEIE SRVRELSKLA ETTDKVKQGF WEEFETLQQQ ECKLLYSRKE GQRQENKNKN RYKNILPFDH TRVVLHDGDP NEPVSDYINA NIIMPEFETK CNNSKPKKSY IATQGCLQNT VNDFWRMVFQ ENSRVIVMTT KEVERGKSKC VKYWPDEYAL KEYGVMRVRN VKESAAHDYT LRELKLSKVG QGNTERTVWQ YHFRTWPDHG VPSDPGGVLD FLEEVHHKQE SIMDAGPVVV HCSAGIGRTG TFIVIDILID IIREKGVDCD IDVPKTIQMV RSQRSGMVQT EAQYRFIYMA VQHYIETLQR RI

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Experimental information

BeamInstrument name: Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2
City: Stanford, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.1127 Å / Dist. spec. to detc.: 1.65 mm
DetectorName: Rayonix MX225-HE
Scan
Title: Protein tyrosine phosphatase SHP2 with E76K activating mutation
Measurement date: Mar 28, 2016 / Storage temperature: 4 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/A /
MinMax
Q0.0141 0.35
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 282 /
MinMax
Q0.014969 0.274025
P(R) point1 282
R0 95.28
Result
Experimental MW: 53.15 kDa / Type of curve: merged
P(R)GuinierGuinier error
Forward scattering, I0143.3 142.7 0.44
Radius of gyration, Rg2.95 nm2.91 nm0.062

MinMax
D-9.53
Guinier point2 34

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