SASDDH3: Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2)

基本情報

• 登録情報: データベース: SASBDB / ID: SASDDH3

試料

Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2)

• Transcriptional regulator Lrs14-like protein (protein), AbfR2, Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

• 機能・相同性: Transcription regulator TrmB, N-terminal / Sugar-specific transcriptional regulator TrmB / helix_turn_helix, Arsenical Resistance Operon Repressor / HTH ArsR-type DNA-binding domain / ArsR-like helix-turn-helix domain / Winged helix DNA-binding domain superfamily / DNA-binding transcription factor activity / Winged helix-like DNA-binding domain superfamily / Transcriptional regulator Lrs14-like protein
• 生物種: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (好気性・好酸性)
• 引用: ジャーナル: Acta Crystallogr D Struct Biol / 年: 2018; タイトル: Crystal structure of an Lrs14-like archaeal biofilm regulator from Sulfolobus acidocaldarius.; 著者: Marian S Vogt / Simon L Völpel / Sonja Verena Albers / Lars Oliver Essen / Ankan Banerjee / ; 要旨: The small winged helix-turn-helix (wHTH) proteins of the Lrs14 family are major transcriptional regulators and act as archaeal biofilm regulators (AbfRs) in the crenarchaeote Sulfolobus acidocaldarius. Here, the first crystal structure of an AbfR ortholog, AbfR2, the deletion of which is known to impair biofilm formation, is presented. Like most other wHTH orthologs, AbfR2 is dimeric in solution as well as in its 2.45 Å resolution crystal structure. Given the presence of three independent AbfR2 dimers in the asymmetric unit, the crystal structure shows a considerable degree of conformational variation within the dimer, the antiparallel orientations of which are stabilized by coiled-coil interaction between H4 helices. Conserved anchor interactions between helices H0 and H4 of AbfR2 further contribute to dimer stabilization. The combined structural and bioinformatic analysis reveals cluster-specific structural differences between different members of the Lrs14 protein family.

登録者

• Marian Vogt (University of Marburg, Marburg, Germany)

モデル

• モデル #1783: ; タイプ: dummy / ダミー原子の半径: 2.10 A / カイ2乗値: 0.812 / P-value: 0.101902; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #1786: ; タイプ: atomic / ダミー原子の半径: 1.90 A / カイ2乗値: 1.052; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2); 試料濃度: 0.83 mg/ml
• バッファ: 名称: 300 mM NaCl, 20 mM HEPES, pH 7.5 / pH: 7.5

要素 #919

名称: AbfR2 / タイプ: protein / 記述: Transcriptional regulator Lrs14-like protein / 分子量: 16.368 / 分子数: 2
由来: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
参照: UniProt: Q4J9G1
配列:

MGSSHHHHHH SQDPNSMETV LQIPYQKKTQ IEKLLEFMYG LNEKEVQLIF RLLYSDTKLN IEELAEEFKV SKALISKSLS ELANKGLIER EKVSNEGRKG RPIYVYYVDR EQLFKRISRD LEELVQASIA KLKEYIFKS

実験情報

• ビーム: 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.124 Å
• 検出器: 名称: Pilatus 1M / タイプ: Dectris / Pixsize x: 172 mm

スキャン

タイトル: Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2)
測定日: 2016年5月5日 / 保管温度: 4 °C / セル温度: 4 °C / 照射時間: 1 sec. / フレーム数: 10 / 単位: 1/nm /

	Min	Max
Q	0.0348	4.9439

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 449 /

	Min	Max
Q	0.171421	2.28206
P(R) point	1	449
R	0	10

結果

カーブのタイプ: single_conc
コメント: The associated atomic model structure is included for comparative purposes only, i.e., to compare the overall topology of the atomic model with the solution scattering and the DAMMIF bead model. Two fits displayed for the atomic model were calculated using CRYSOL (top) and SASREF (bottom).

	実験値	Standard	Porod
分子量	39 kDa	39.29 kDa	37 kDa
体積	-	-	56.89 nm3

	P(R)	Guinier	Guinier error
前方散乱 I0	24.03	24.16	0.09
慣性半径, Rg	2.8 nm	2.85 nm	0.03

	Min	Max
D	-	10
Guinier point	16	72