PDB-7ox9: Target-bound SpCas9 complex with AAVS1 all-RNA guide

ID or keywords:

Entry

Database: PDB / ID: 7ox9

Title

Target-bound SpCas9 complex with AAVS1 all-RNA guide

Components

AAVS1 non-target DNA strand
AAVS1 target DNA strand
CRISPR-associated endonuclease Cas9/Csn1
sgRNASubgenomic mRNA

Keywords

HYDROLASE /

Cas9 /

CRISPR /

sgRNA / AAVS1 /

nuclease

Function / homology

Function and homology information

maintenance of CRISPR repeat elements / 3'-5' exonuclease activity / DNA endonuclease activity / defense response to virus /

Hydrolases; Acting on ester bonds /

DNA binding /

RNA binding /

metal ion binding
Similarity search - Function

Biological species

Streptococcus pyogenes serotype M1 (bacteria)
synthetic construct (others)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.45 Å

Authors

Pacesa, M. / Donohoue, P. / May, A.P. / Jinek, M. / Cameron, P.

Funding support

Switzerland, 1items

Citation

Journal: Mol.Cell / Year: 2021
Title: Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells.
Authors: Donohoue, P.D. / Pacesa, M. / Lau, E. / Vidal, B. / Irby, M.J. / Nyer, D.B. / Rotstein, T. / Banh, L. / Toh, M.S. / Gibson, J. / Kohrs, B. / Baek, K. / Owen, A.L.G. / Slorach, E.M. / van ...

History

Deposition	Jun 22, 2021	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Sep 15, 2021	Provider: repository / Type: Initial release
Revision 1.1	Jan 31, 2024	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	7ox9.cif.gz	848.6 KB	Display	PDBx/mmCIF format
PDB format	pdb7ox9.ent.gz	574.6 KB	Display	PDB format
PDBx/mmJSON format	7ox9.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/ox/7ox9 ftp://data.pdbj.org/pub/pdb/validation_reports/ox/7ox9	HTTPS FTP

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Related structure data

Related structure data	7ox7C 7ox8C 7oxaC 5fq5S S: Starting model for refinement C: citing same article (ref.)
Similar structure data	6k4q-d 5fw2-d 5fw3-d 6k4p-d 5b2t-d 5fq5-d 7ox7-d 5b2r-d 7ox8-d 6k57-d 6k4u-d 7oxa-d 5b2s-d 4un5-d 4un3-d Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#181 - Jan 2015 Cascade and CRISPR similarity (16) #253 - Jan 2021 Expressome similarity (2) #121 - Jan 2010 70S Ribosomes similarity (1) #16 - Apr 2001 Aminoacyl-tRNA Synthetases similarity (1) #130 - Oct 2010 Riboswitches similarity (1) #157 - Jan 2013 Transfer-Messenger RNA similarity (1) #67 - Jul 2005 TATA-Binding Protein similarity (1) #229 - Jan 2019 Fluorescent RNA Aptamers similarity (1) #168 - Dec 2013 DNA Helicase similarity (1) #87 - Mar 2007 Zinc Fingers similarity (2) #91 - Jul 2007 Thymine Dimers similarity (1) #260 - Aug 2021 Ribonuclease P similarity (1) #255 - Mar 2021 Cisplatin and DNA similarity (1) #249 - Sep 2020 SARS-CoV-2 RNA-dependent RNA Polymerase similarity (1) #146 - Feb 2012 Aminoglycoside Antibiotics similarity (1) #172 - Apr 2014 RecA and Rad51 similarity (1) #227 - Nov 2018 Telomerase similarity (2) #122 - Feb 2010 Enhanceosome similarity (1) #15 - Mar 2001 Transfer RNA similarity (1) #84 - Dec 2006 Transposase similarity (1) #48 - Dec 2003 Catabolite Activator Protein similarity (1) #10 - Oct 2000 Ribosome similarity (1) #210 - Jun 2017 Adenine Riboswitch in Action similarity (1) #150 - Jun 2012 Sliding Clamps similarity (1) #135 - Mar 2011 Integrase similarity (1) #112 - Apr 2009 Oct and Sox Transcription Factors similarity (1) #180 - Dec 2014 TAL Effectors similarity (1) #65 - May 2005 Self-splicing RNA similarity (1) #105 - Sep 2008 Ribonuclease A similarity (1) #226 - Oct 2018 Aminoglycoside Antibiotics and Resistance similarity (1) #245 - May 2020 Spliceosomes similarity (1) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (1) #98 - Feb 2008 Small Interfering RNA similarity (1) #73 - Jan 2006 Topoisomerase similarity (1) #40 - Apr 2003 RNA Polymerase similarity (1) #139 - Jul 2011 DNA Methyltransferase similarity (1) #155 - Nov 2012 Vitamin D Receptor similarity (1) #3 - Mar 2000 DNA Polymerase similarity (1) #161 - May 2013 Ricin similarity (1) #89 - May 2007 Aconitase and Iron Regulatory Protein 1 similarity (1) #81 - Sep 2006 Elongation Factors similarity (1) #119 - Nov 2009 Designed DNA Crystal similarity (1) #266 - Feb 2022 Oligosaccharyltransferase similarity (1) #125 - May 2010 Parvoviruses similarity (1) #131 - Nov 2010 Inteins similarity (1) #230 - Feb 2019 Initiation Factor eIF4E similarity (1) #160 - Apr 2013 Actinomycin similarity (1) #190 - Oct 2015 Two-component Systems similarity (1) #23 - Nov 2001 DNA similarity (1) #7 - Jul 2000 Nucleosome similarity (1) #240 - Dec 2019 Hypoxia-Inducible Factors similarity (1) #8 - Aug 2000 Restriction Enzymes similarity (1) #257 - May 2021 Fetal Hemoglobin similarity (1) #258 - Jun 2021 Glucocorticoid Receptor and Dexamethasone similarity (1) #143 - Nov 2011 Toll-like Receptors similarity (1) #31 - Jul 2002 p53 Tumor Suppressor similarity (1) #106 - Oct 2008 Poly(A) Polymerase similarity (1) #33 - Sep 2002 HIV Reverse Transcriptase similarity (1) #104 - Aug 2008 Selenocysteine Synthase similarity (1) #39 - Mar 2003 lac Repressor similarity (1) #92 - Aug 2007 Anabolic Steroids similarity (1) #45 - Sep 2003 Estrogen Receptor similarity (1) #55 - Jul 2004 DNA Ligase similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (2)

PDB pages

PDBj /

wwPDB /

NCBI

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Deposited unit

A: sgRNA

B: CRISPR-associated endonuclease Cas9/Csn1

C: AAVS1 target DNA strand

D: AAVS1 non-target DNA strand

hetero molecules

199 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software
Evidence: native gel electrophoresis

Unit cell

Length a, b, c (Å)	178.080, 68.205, 187.771
Angle α, β, γ (deg.)	90.000, 111.144, 90.000
Int Tables number	5
Space group name H-M	C121
Space group name Hall	C2y
Symmetry operation	#1: x,y,z #2: -x,y,-z #3: x+1/2,y+1/2,z #4: -x+1/2,y+1/2,-z

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DNA chain , 2 types, 2 molecules CD

#3: DNA chain	AAVS1 target DNA strand Mass: 8446.445 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#4: DNA chain	AAVS1 non-target DNA strand Mass: 3748.449 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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RNA chain / Protein , 2 types, 2 molecules AB

#1: RNA chain	sgRNA / Subgenomic mRNA Mass: 27290.303 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#2: Protein	CRISPR-associated endonuclease Cas9/Csn1 / SpCas9 / SpyCas9 Mass: 158875.031 Da / Num. of mol.: 1 / Mutation: D10A, H840A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus pyogenes serotype M1 (bacteria) Gene: cas9, csn1, SPy_1046 / Plasmid: pMJ841 / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta 2(DE3) References: UniProt: Q99ZW2, Hydrolases; Acting on ester bonds

#1: RNA chain

sgRNA /

Subgenomic mRNA

Mass: 27290.303 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

#2: Protein

CRISPR-associated endonuclease Cas9/Csn1 / SpCas9 / SpyCas9

Mass: 158875.031 Da / Num. of mol.: 1 / Mutation: D10A, H840A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Streptococcus pyogenes serotype M1 (bacteria)
Gene: cas9, csn1, SPy_1046 / Plasmid: pMJ841 / Production host:

Escherichia coli (E. coli) / Strain (production host): Rosetta 2(DE3)
References: UniProt: Q99ZW2,

Hydrolases; Acting on ester bonds

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Non-polymers , 3 types, 500 molecules

#5: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#6: Chemical	ChemComp-K / POTASSIUM ION Mass: 39.098 Da / Num. of mol.: 11 / Source method: obtained synthetically / Formula: K
#7: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 487 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.68 Å³/Da / Density % sol: 54.12 %
Crystal grow	Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 0.1 M Tris-acetate pH 8.5, 0.3-0.5 M KSCN, 17-19% PEG3350

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1 Å
Detector	Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Oct 22, 2017
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 2.45→48.16 Å / Num. obs: 77217 / % possible obs: 99.2 % / Redundancy: 6.7 % / B_iso Wilson estimate: 53.04 Å² / CC_1/2: 0.997 / R_merge(I) obs: 0.14 / Net I/σ(I): 10.2
Reflection shell	Resolution: 2.45→2.54 Å / Redundancy: 6.4 % / R_merge(I) obs: 1.606 / Mean I/σ(I) obs: 1.04 / Num. unique obs: 7600 / CC_1/2: 0.493 / % possible all: 98.69

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Processing

Software

Name	Version	Classification
Coot	0.8.9	model building
PHENIX	1.17.1_3660	refinement
XDS		data reduction
XSCALE		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 5FQ5

5fq5

Resolution: 2.45→48.16 Å / SU ML: 0.3871 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 27.2962
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2326	3863	5.01 %
R_work	0.1948	73317	-
obs	0.1968	77180	99.25 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 66.89 Å²

Refinement step

Cycle: LAST / Resolution: 2.45→48.16 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	10930	2540	13	487	13970

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0033	13966
X-RAY DIFFRACTION	f_angle_d	0.6259	19360
X-RAY DIFFRACTION	f_chiral_restr	0.0394	2206
X-RAY DIFFRACTION	f_plane_restr	0.004	2037
X-RAY DIFFRACTION	f_dihedral_angle_d	18.7852	5661

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.45-2.48	0.3758	145	0.3638	2535	X-RAY DIFFRACTION	98.53
2.48-2.51	0.3162	126	0.3292	2639	X-RAY DIFFRACTION	98.75
2.51-2.54	0.3775	138	0.3217	2532	X-RAY DIFFRACTION	98.67
2.54-2.58	0.3375	132	0.2997	2599	X-RAY DIFFRACTION	97.71
2.58-2.62	0.3429	141	0.2864	2590	X-RAY DIFFRACTION	99.6
2.62-2.66	0.343	136	0.2859	2608	X-RAY DIFFRACTION	99.6
2.66-2.7	0.3138	137	0.2741	2610	X-RAY DIFFRACTION	99.67
2.7-2.74	0.387	138	0.2673	2610	X-RAY DIFFRACTION	99.85
2.74-2.79	0.2931	144	0.2583	2627	X-RAY DIFFRACTION	99.93
2.79-2.84	0.3063	131	0.2528	2637	X-RAY DIFFRACTION	99.93
2.84-2.89	0.2806	139	0.2436	2607	X-RAY DIFFRACTION	99.89
2.89-2.95	0.311	141	0.2544	2624	X-RAY DIFFRACTION	99.82
2.95-3.02	0.3016	136	0.2548	2600	X-RAY DIFFRACTION	99.71
3.02-3.09	0.287	138	0.2518	2654	X-RAY DIFFRACTION	99.79
3.09-3.16	0.259	138	0.2292	2609	X-RAY DIFFRACTION	99.64
3.16-3.25	0.2623	139	0.2155	2598	X-RAY DIFFRACTION	99.35
3.25-3.34	0.2667	136	0.2013	2601	X-RAY DIFFRACTION	99.24
3.35-3.45	0.2614	135	0.201	2626	X-RAY DIFFRACTION	99.07
3.45-3.58	0.2562	140	0.1945	2614	X-RAY DIFFRACTION	98.89
3.58-3.72	0.2434	135	0.1877	2551	X-RAY DIFFRACTION	97.6
3.72-3.89	0.1888	139	0.1726	2608	X-RAY DIFFRACTION	99.21
3.89-4.09	0.1828	138	0.1618	2661	X-RAY DIFFRACTION	99.64
4.09-4.35	0.1987	139	0.1529	2636	X-RAY DIFFRACTION	99.36
4.35-4.69	0.1779	137	0.1393	2630	X-RAY DIFFRACTION	99.64
4.69-5.16	0.1821	142	0.1459	2651	X-RAY DIFFRACTION	99.68
5.16-5.9	0.2075	141	0.1635	2673	X-RAY DIFFRACTION	99.43
5.9-7.43	0.2105	138	0.1813	2632	X-RAY DIFFRACTION	97.67
7.43-48.16	0.1722	144	0.165	2755	X-RAY DIFFRACTION	99.31

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.638690624628	-0.44930357545	0.420401266101	0.355720991339	0.245812430485	2.66303810815	-0.223423405681	-0.105217861401	0.0374768560597	0.0573584281462	0.160993794486	0.125308755714	0.718754823835	-0.54789894117	0.0567111639081	0.508582848694	-0.0798080619917	0.0678913968006	0.407091103879	-0.0503576466977	0.508371302166	-7.14486721503	6.36588451609	61.0229265621
2	0.844755908461	-0.103569355786	0.166402937323	1.18521615542	0.49973806853	0.725588364902	0.341523095895	0.742787635055	0.0870122414149	-0.202987619902	0.187117098301	-0.335109353096	1.03322300977	0.444349100757	-0.0474020715657	0.700108323767	0.192586533393	-0.0746732912555	0.898772047404	-0.164401223469	0.435091091721	18.3055093016	-5.50366814618	13.340983642
3	3.27942147724	0.425150023512	0.434799097218	1.83721056375	0.0491773604864	2.85712552396	0.541403916615	0.844178427788	-0.0752343433185	-0.9346774301	0.000325997473069	0.648464410528	0.459494535464	0.119663728745	-0.47141162262	1.42316394607	0.273550170377	-0.274692261556	1.3339348841	-0.294109999209	0.989873072352	19.3310277531	-19.7769313355	3.16173784678
4	1.3742957067	0.495714769272	0.589466315367	0.490094140082	0.0967802709749	1.47486317692	0.0813922640605	0.174574801331	0.099979737361	-0.119107324358	-0.0257494002367	0.074745088244	-0.0363221218504	0.168573423889	-0.128474593264	0.354708042859	-0.0244147330913	-0.0220426437094	0.338359760666	0.0106799167023	0.34437510837	-1.04969180352	8.56866586213	29.1730566877
5	0.206872539434	-0.422337320716	-0.022076836494	1.05506730541	0.692897327928	2.09874973853	0.657366077259	-0.230079290156	1.58763951439	-0.214682778612	-0.0420961890967	-0.40894741244	-2.03799581651	1.08540958457	-0.315888405713	1.20395510931	-0.221385815728	0.241216410186	0.828064533137	0.236039031411	1.44989302946	-38.8293593315	16.9165401719	29.4190263984
6	2.93667607403	2.19784256622	-0.992800306754	2.99408456995	-1.21466208346	0.893359761279	0.470358732186	-1.01191390158	0.349326679283	-0.0413775647756	-0.0407874116227	-0.109569802984	-1.46766359502	0.506677679585	-0.302999635266	1.09957299251	-0.261569923574	0.204985727338	1.28487108665	-0.138906509941	0.852861664638	-39.9165790625	14.2103137546	37.4150948197
7	0.374710581593	0.0876848665812	0.22121196993	0.280490442433	-0.0217176298593	1.15683445992	0.0903078024017	0.121208494588	-0.0878189588383	0.139782781885	-0.0527291989981	-0.108674265819	0.166702000213	0.492716781096	-0.0496380726617	0.445639393336	0.0281978293737	-0.0409645655525	0.485877590679	-0.0373693560115	0.46357141209	15.393505856	-0.546043629837	42.5212916845
8	0.49948355512	0.0680822708768	0.0903163933411	0.314456799469	-0.0656281691228	2.43193910543	-0.088410159024	-0.118155272675	0.03691855995	0.20312392501	0.0733119046714	-0.0487223819423	-0.403390658989	0.0159591860249	0.0519949799487	0.536875584378	0.0382506224002	-0.0124515890436	0.250208744742	-0.0190632035002	0.431484416459	0.378548596019	20.3689078894	69.4033933021
9	0.669645052706	-0.920411845207	0.497886962826	1.20772734504	-0.489077683662	1.4932219451	0.111641194238	-0.00533722418605	-0.0726951817578	0.0102374150088	-0.0653074853331	-0.245020867861	0.49821729529	0.249249776554	-0.102534042189	0.569699637826	-0.0304018568702	-0.00495343820898	0.44442998287	-0.0365740056489	0.62119221008	-7.51739862944	-14.7934618823	64.1443750328
10	1.27090395586	0.200676516031	-0.336728644178	0.375722146264	-0.0841030644257	1.5059534725	-0.0979301641371	0.0266025045912	-0.202564556524	0.0517348681532	0.068333374365	0.0413318284289	0.461595834932	-0.294315993093	0.016508541098	0.512975338754	-0.135179139783	0.028720821117	0.406644824291	-0.0294838659163	0.498853326744	-29.4285980528	-12.4882230993	43.6707900948
11	0.87600386827	-0.0266096175905	-0.171196984931	0.200690587449	-0.664264551996	2.26522513709	0.247933468875	0.250370726657	-0.877981974745	-0.28683496705	0.231574895697	0.315976213505	0.476658984865	-1.19666641845	0.056890435956	1.32202681244	-0.676081475362	-0.0702788691907	1.78792923073	-0.452004133601	0.988285100177	-15.765087749	-21.322263279	19.0168749386
12	2.03399183317	0.955842629887	0.0531605033449	1.18427670947	-0.509301766931	0.39558247983	-0.0171920171593	-0.45873935298	-0.425606607024	0.22087591647	0.0203199335457	-0.162291695277	0.207929971601	0.340114541619	-0.0139297381302	0.505312972803	-0.108437814734	-0.0125329716622	0.477172019478	-0.106893089858	0.544706094367	-2.58909014998	-9.67290877062	31.1845158845
13	2.42836151728	0.0918102945678	-0.870989476636	0.658965173313	-0.479910281489	1.5785243723	0.21163832908	-0.0377436221194	0.321945473058	0.34600764628	0.0964080776131	-0.0473075248301	0.181790032741	-0.231648816506	-0.34356510866	0.5022371212	-0.108796998966	0.00136917422562	0.384725893622	0.0296648665502	0.395390725532	-1.00796227026	6.92276302281	55.0741740008
14	1.22442471009	-0.258451809911	-0.0315983423167	0.703670853245	-0.0793477749121	2.13501378421	-0.263253785159	-0.13103567028	-0.143765260206	0.249436427856	0.213795321671	0.498798183834	0.724046209967	-0.956774356138	-0.100804903737	0.686301967799	-0.0412101692144	0.0907348756697	0.649404213641	0.063831450307	0.560459361831	-14.5789601533	2.61568780863	78.0958930291
15	4.23518910798	-0.0106545441336	-0.994229820463	5.98854870513	0.585617484071	2.69683283563	-0.0377585911745	0.441272736575	-1.04818798914	0.182971829661	-0.0553417492978	-0.00663017623353	1.19441578014	0.114042717256	-0.0209006130783	0.617597888528	-0.0743033980408	-0.0015486832977	0.493309798249	-0.177135365295	0.695358553239	-11.6585352783	-14.6246225384	24.0642525731

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 0 through 19 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 20 through 34 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 35 through 39 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 40 through 69 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 70 through 74 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 75 through 82 )
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 3 through 411 )
8	X-RAY DIFFRACTION	8	chain 'B' and (resid 412 through 730 )
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 731 through 905 )
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 906 through 1367 )
11	X-RAY DIFFRACTION	11	chain 'C' and (resid -7 through -3 )
12	X-RAY DIFFRACTION	12	chain 'C' and (resid -2 through 2 )
13	X-RAY DIFFRACTION	13	chain 'C' and (resid 3 through 12 )
14	X-RAY DIFFRACTION	14	chain 'C' and (resid 13 through 20 )
15	X-RAY DIFFRACTION	15	chain 'D' and (resid -2 through 7 )

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